Comparative adsorption of natural lung surfactant, extracted phospholipids, and artificial phospholipid mixtures to the air-water interface
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Suppression of L<inf>α</inf>/L<inf>β</inf> Phase Coexistence in the Lipids of Pulmonary Surfactant
2021, Biophysical JournalCitation Excerpt :HPLC grade chloroform, trifluoroethanol, and methanol were obtained from Thermo Fischer Scientific (Pittsburgh, PA). The constituents of calf lung surfactant extract (CLSE), provided by ONY Biotech (Amherst, NY), were obtained by extracting (25) phospholipid aggregates lavaged from freshly excised calf lungs (26). Subfractions of the constituents in CLSE were obtained by established methods (27) based on gel permeation chromatography (28,29).
Adsorption of Phospholipids at the Air-Water Surface
2019, Biophysical JournalCitation Excerpt :Adsorption is considered to reach an equilibrium when the lipid molecules are closely packed at the surface and reach a dynamic equilibrium with vesicles in the bulk phase (21,22). Despite numerous experimental studies of lipid adsorption (22–26), the detailed biophysical mechanism of lipid vesicle adsorption is still unclear. Various experimental methodologies, including Langmuir balance (12), pulsating bubble surfactometry (27), captive bubble surfactometry (22), micropipette technique (28), and infrared reflection-absorption spectroscopy (25), have been used for measuring the dynamic surface tension and the structure of adsorbed phospholipid films.
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2010, Biophysical JournalCitation Excerpt :POPE was obtained from Avanti Polar Lipids (Alabaster, AL) and used without further characterization or purification. Extracts of pulmonary surfactant from calf lungs (ONY, Amherst, NY) were prepared as described previously (22). Hydrophobic SPs were obtained from the extracted surfactant by minor modifications of a previously described protocol based on gel permeation chromatography (23).
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