RT Journal Article SR Electronic T1 A clinicians’ review of the respiratory microbiome JF Breathe JO Breathe FD European Respiratory Society SP 210161 DO 10.1183/20734735.0161-2021 VO 18 IS 1 A1 Christina D. Campbell A1 Clea Barnett A1 Imran Sulaiman YR 2022 UL http://breathe.ersjournals.com/content/18/1/210161.abstract AB The respiratory microbiome and its impact in health and disease is now well characterised. With the development of next-generation sequencing and the use of other techniques such as metabolomics, the functional impact of microorganisms in different host environments can be elucidated. It is now clear that the respiratory microbiome plays an important role in respiratory disease. In some diseases, such as bronchiectasis, examination of the microbiome can even be used to identify patients at higher risk of poor outcomes. Furthermore, the microbiome can aid in phenotyping. Finally, development of multi-omic analysis has revealed interactions between the host and microbiome in some conditions. This review, although not exhaustive, aims to outline how the microbiome is investigated, the healthy respiratory microbiome and its role in respiratory disease.Educational aimsTo define the respiratory microbiome and describe its analysis.To outline the respiratory microbiome in health and disease.To describe future directions for microbiome research.The respiratory microbiome encompasses bacterial, fungal and viral communities. In health, it is a dynamic structure and dysbiotic in disease. Dysbiosis can be related to disease severity and may be utilised to predict patients at clinical risk. https://bit.ly/3pNSgnA