PT  - JOURNAL ARTICLE
AU  - Vasiliu, Anca
AU  - Saktiawati, Antonia Morita Iswari
AU  - Duarte, Raquel
AU  - Lange, Christoph
AU  - Cirillo, Daniela Maria
ED  - ,
TI  - Implementing molecular tuberculosis diagnostic methods in limited-resource and high-burden countries
AID  - 10.1183/20734735.0226-2022
DP  - 2022 Dec 01
TA  - Breathe
PG  - 220226
VI  - 18
IP  - 4
4099  - http://breathe.ersjournals.com/content/18/4/220226.short
4100  - http://breathe.ersjournals.com/content/18/4/220226.full
SO  - Breathe2022 Dec 01; 18
AB  - Tuberculosis (TB) is one of the deadliest infectious diseases in the world with more than a million people dying of TB each year. Accurate and timely TB diagnosis has the potential to alleviate the global TB burden; therefore, one of the pillars of the End TB Strategy developed by the World Health Organization (WHO) is the early diagnosis of TB, including universal drug-susceptibility testing (DST). The WHO emphasises the importance of DST before treatment initiation, using molecular WHO-recommended rapid diagnostic tests (mWRDs). Currently available mWRDs are nucleic acid amplification tests, line probe assays, whole genome sequencing, and targeted next-generation sequencing. However, implementing the sequencing mWRDs in routine laboratories in low-income countries is constrained by the existing infrastructure, high cost, the specialised skills needed, data storage, and the current delay in results compared with other routine methods. These limitations are pronounced in resource-limited settings, which often have a high TB burden and need for innovative TB diagnostic technologies. In this article we propose several possible solutions, like adapting infrastructure capacity to needs, advocating for lowering costs, building bioinformatics and laboratory capacity, and increasing the use of open-access resources for software and publications.Next-generation sequencing has the potential to drastically change the landscape of tuberculosis diagnosis worldwide. Considerate implementation in low-resource settings should ensure access and sustainability, especially through new portable platforms. https://bit.ly/3iiWFyo