Basic and clinical immunologyCytokine production by bronchoalveolar lavage T lymphocytes in chronic obstructive pulmonary disease
Section snippets
Subjects
The study protocol was approved by the ethics committees of the Silesian Medical Academy and The Royal Brompton and Harefield Hospital. All subjects provided written informed consent before entering the study.
Eleven patients with moderate stable COPD (7 male and 4 female patients) were recruited for the BAL study, and 4 patients were recruited for the blood study. The inclusion criteria were as follows: FEV1/forced vital capacity ratio of less than 70%, FEV1 of less than 85% of predicted value,
Patient and BAL cell characterization
The total and differential BAL cell counts obtained from the 2 subject groups are shown in Table II. Recovery of BAL fluid was significantly lower in patients with COPD compared with that seen in control subjects, although the total number of cells recovered was no different between the 2 groups. The number of CD8+ (median, 0.8 × 105; range, 0.3-21.5 × 105) and CD4+ (median, 1.28 × 105; range, 0.15-10 × 105) T cells isolated from all subjects was similar. The number of CD8+ (P = .01), but not
Discussion
In this study we demonstrate the profile of cytokine production by CD8+ and CD4+ T cells isolated from the BAL fluid of patients with COPD for the first time. IL-4 was expressed by a higher percentage of stimulated CD8+ T cells compared with CD4+ T cells in patients with COPD. In contrast, IFN-γ was expressed in a significantly higher percentage of stimulated CD4+ T cells (TH1) than CD8+ T cells (TC1) in the COPD group. TNF-α was expressed by almost all IFN-γ+ cells (TC1 and TH1 cells), with
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Supported by a research grant from GlaxoSmithKline (UK). AB was an ERS and Polish Foundation of Science Fellow.
Disclosure of potential conflict of interest: O. Kon is on advisory panels for GlaxoSmithKline and AstraZeneca. The rest of the authors have declared that they have no conflict of interest.
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Dr Barczyk is currently affiliated with the Department of Pneumology, Silesian Medical Academy, Katowice, Poland.