Chest
Original ResearchInherited Lung DiseasesCiliated Air-Liquid Cultures as an Aid to Diagnostic Testing of Primary Ciliary Dyskinesia
Section snippets
Patients
Between April 1, 2007, and March 31, 2008, 231 patients were referred to the PCD diagnostic service in Leicester. This center is one of the three national PCD diagnostic centers in England, all of which are funded to carry out cell culture routinely on clinical samples. This report is an audit of results of air-liquid interface cultures of patients referred for diagnostic testing of PCD in Leicester. All patients gave their consent for participating in the PCD service, and all samples were
Results
Between April 1, 2007, and March 31, 2008, 231 patients were referred for diagnostic testing for PCD. Of the patients referred, 217 had a brush biopsy performed.
Discussion
Although we found cell culture with redifferentiation of the ciliated epithelium helpful in a number of patients, it is a highly specialized and time-consuming method. A major advantage was that, when successful, this technique allowed detailed evaluation of both ciliary beat pattern and electron microscopy. In our hands only 54% of biopsy samples could be cultured to a ciliated phenotype. This is likely to be due to a number of factors. First, our brushing technique obtains relatively few
Acknowledgments
Author contributions: Dr Hirst explicitly declares that he has had access to and takes responsibility for the integrity of the data and the accuracy of the data analysis.
Dr Hirst: contributed to writing the manuscript, analyzing the data, and developing the cell culture method.
Mr Rutman: contributed to performing the electron microscopy.
Ms Williams: contributed to culturing the cells.
Dr O'Callaghan: contributed to preparing the manuscript and is the clinical leader of the PCD service in
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Funding/Support: This study was funded by the National Commissioning Group via the National Health Service, England.
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